Liquid Liquid Equilibrium of Pold (Ethylene Glycol)

Liquid Liquid Equilibrium of Pold (Ethylene Glycol) LiquidLiquid Equilibrium of Poly (ethylene glycol) 1500 + di-Potassium Tartrate +Water at different pH (6.41, 7.74 and 9.05) Alireza Barani Chemical Engineering Department,Faculty of Engineering, Shomal University, Amol, PO Box 731, Iran Mohsen Pirdashti[1] Chemical Engineering Department,Faculty of Engineering, Shomal University, Amol, PO Box 731, Iran Abbas Ali Rostami Chemical Engineering Department,Faculty of Engineering, Shomal University, Amol, PO Box 731, Iran Abstract: Liquid liquid equilibrium (LLE) data have been determined for aqueous two-phase systems (ATPSs) containing (ATPS) poly (ethylene glycol) (PEG) 1500 +di potassium tartrate +water at 298.15 K and in various pH values (6.41, 7.74 and 9.05). Two physical properties (density and refractive index) were used to obtain the compositions of phase and the ends of the tie-lines. The effect of pH on the binodal curve, tie-line length and slope of tie line are discussed. The binodal curves of these systems have been correlated by Bleasdales equation. Furthermore, the Othmer-Tobias and Bancroft equations was used to correlate the tie line data points. Finally, the effective excluded volume (EEV) of the salt into the PEG aqueous solution were obtained. Keywords: ATPS; Phase diagram; pH; Refractive index; Poly(ethylene glycol); di potassium tartrate Introduction The dissolving of one polymer and one salt or two aqueous polymer solutions together in water results in the formation of two immiscible aqueous phases systems, called Aqueous Two-Phase Systems (ATPSs). Albertson introduced these systems in 1965 for the purpose of separating the biological materials1. Several industries can benefit from employing ATPS including biotechnology, petroleum, paint, adhesives, and pharmaceuticals 2, 3. Moreover, the ATPS is effective in providing separation technique due to its undemanding scale-up viability 4-6, economic efficiency7, 8, ease of continuous process 9, decreased interfacial tension 10, short processing time 11, low energy consumption 12, 13, good resolution 14, high yield 15, relatively high load capacity 16, and selective extraction 17. The data derived from phase diagram, composition and the physical properties of the phase formation are essential in order to optimize, design and increase the size of these processes; and develop the models that predict phase partitioning18-20. Poly Ethylene Glycol (PEG) is a water-soluble hydrophilic and biocompatible polymer employed by the studies about ATPS 21. Accordingly, Selber et al. (2004) 3 provided a useful summary of experimental liquid-liquid data and equilibrium diagrams for systems including PEG, inorganic salts and water. Peng et al.10 (1995) investigated the phase diagram and protein partition coefficient in ATPS containing PEG and K2HPO4 + KH2PO4 and found some merits in this polymer-salt system. Furthermore, several studies 22-27 have focused on the Liquid-Liquid Equilibrium (LLE) data of PEG + salt ATPSs. Zafarani-Moattar et al. (2008) indicated some advantages of using tartrate such as biodegradability and effectiveness in partitioning of biological materials through being discharged into biological waste water treatment plants 27. In the current study, the phase equilibrium data for PEG1500 +di-potassium tartrate (K2C4H4O6 ) +H2O were determined at 298.15K and th ree pH values (6.41 , 7.74, and 9.05). In addition, the effects of pH on the binodal curve and Tie-Line Length (TLL) and Slope of Tie Line (STL) were determined. Likewise, the calibration curves were applied as an analytical technique [MN1]with measuring the density and refractive index. Finally, Othmer-Tobias and Bancroft equations 28 were used to fit the tie- line data and Bleasdales equation was employed 29 to correlate the experimental LLE data from the investigated systems. Experimental Materials To prepare the materials, PEG [HO (C2H4O) n H] with average of 1500 gmol-1 and di potassium tartrate with minimum purity of 99.5% by mass were obtained from Merck. The polymer and salts were used without further purification with the distilled deionized water. 2.2. Apparatus and Procedure. 2.2.1. Analytical Methods The same method of calibration plots and evaluation of parameters in the literatures 30 were employed to obtain the compositions in both phases from measurements of the two physical properties (density and refractive index) at 298.15 K. in order to obtain the compositions, calibration equations were previously obtained. Homogeneous ternary mixtures with compositions from 0 to 30 wt% (total solute composition) were prepared by weight, and then density and refractive index were measured at 298.15 K. the concentration of PEG and salt were obtained using eq 1, which related the refractive index and density to the concentration of salt and PEG at 298.15 K, where represents the mass fraction of PEG, is the mass fraction of di-potassium tartrate, and is the value of the refractive index and density of pure water at 298.15 K. Experimental data were fitted to polynomial expansions up to order 2 by least-squares (order 3 was proved unnecessary in all cases 23: (1) Where Z is the physical property (density or refractive index) and to are fitting parameters. The refractive index was determined by refractive index measurements at 298.15 K using a refractometer (CETI Belgium model) with an accuracy of 0.0001. Then, densities was measured by using an Anton Paar oscillation U-tube densitometer (model: DMA 500) with a precision of  ±10-4 g.cm-3. 2.2.2. Binodal Curve The experimental apparatus employed is similar to the one used previously 31. A glass vessel, volume of 25 cm3 was used to carry out the equilibrium determination. It was provided with an external jacket containing water at constant temperature. The temperature was controlled to within +-0.05 K. The binodal curves were determined by the cloud-point method 32. The cloud-point method was investigated by titration method where step by step and exactly known amounts of polymer (titrant) was added to an aqueous solution salt of known concentration (or vice versa) under stirring until the solution becomes cloudy. 2.2.3. The TLL and STL Tie lines were also determined using the equilibrium set designed by ourselves and according to previously described procedures [14]. For the determination of the tie lines, we selected 4 samples for each pH that were prepared by mixing appropriate amounts of PEG, salt, and water in the vessels. Samples were stirred for 5 min and settled for 24 h, with temperature controlling condition, to ensure that equilibrium was established. To separate the resulting phases, the tubes were centrifuged (Hermle Z206A, Germany) at 6000 rpm for 5 min. The resulted phases showed no turbidity and the top and bottom samples were easily separated. After the equilibrium was achieved, phases were with- drawn using syringes. The top phase was sampled first, with care being taken to leave a layer of material at least 0.5 cm thick above the interface. The bottom phase was remain in the glass vessel with a long needle. TLL provides an empirical measurement of the compositions of the two phases, which can be c alculated by the following equation: TLL= (2) Where and denote the concentration of PEG and salt in top and bottom phase, and STL is given by the ratio of the difference between the polymer and salt concentrations in the top and bottom phases as presented in Eq. 3: STL= (3) Where and are the polymer and salt concentrations, expressed in mass percent, respectively, and the superscripts T and B designate the top and bottom phases, respectively. 2.2.4.Binodal Curve and TLL Correlation For the binodal data correlation, the Bleasdales equation [27] can be suitably used to reproduce the binodal curves of the investigated systems (4) Where a, b, and c represent the fitting parameters and and demonstrate the polymer and salt mass fractions, respectively. The binodal data of the above expression were correlated by least-squares regression. The reliability of the measured tie-line compositions was ascertained by Othmer-Tobias (Eq. 5) and Bancroft (Eq. 6) correlation equations (5) (6) Where is the mass fraction of polymer in the top phase, is the mass fraction of salt in the bottom phase, and are the mass fractions of water in the bottom and top phases, respectively, and , , , and are the adjusted parameters. Besides, the obtained experimental data can also adapt to the equation provided by Guan and co-workers33 Ln (.WPEG/ ) + ./ = 0 (7) Where and stand for the polymer and salt molecular weight, respectively. Moreover, V* is the Effective Excluded Volume (EEV) of the salt in the PEG aqueous solution. Results and Discussion Fitting parameters of calibration equation The values of the coefficients a, b, c, d, e and f for the system studied are shown in table 1, respectively. Table 1.The value of the coefficients observed from eq. 1. 1.3341 0.0581 0.1302 -0.0718 0.2257 0.3882 à Ã‚ /g.cm3 0.9842 0.6783 0.1761 0.0098 0.1643 0.1018 Binodal Curve The binodal curve data of the PEG + di-potassium tartrate + H2O system are presented in Table 2. Table 2. Binodal curve data of the PEG 1500 + di-potassium tartrate+ water system at 298.15 K and 0.1 MPa at different pH values 42.71 9.01 27.75 10.49 43.43 10.49 39.57 9.50 45.58 7.20 43.67 7.20 35.50 10.21 42.04 7.80 41.88 7.80 34.48 10.32 39.31 8.30 39.51 8.30 28.54 11.58 35.77 9.01 28.93 9.01 33.70 10.55 23.88 12.01 22.04 12.2 23.17 13.01 16.18 14.77 19.89 14.77 17.07 15.01 13.94 15.73 16.53 15.73 14.25 16.21 13.42 16.05 14.53 16.05 12.11 17.02 11.75 16.91 12.55 16.91 11.28 17.52 7.59 19.71 11.16 19.71 7.01 21.01 6.90 20.36 9.19 20.36 6.17 21.64 5.29 23.19 8.10 23.19 5.85 22.01 5.03 22.24 7.45 22.24 5.06 27.53 4.74 24.01 6.76 24.01 4.65 23.50 4.32 24.70 5.80 24.70 3.17 26.01 3.62 26.01 4.99 26.02 Standard uncertainties: u(wi) = 0.002; u(P) = 5 kPa; u(T) = 0.05 K. Figure 1 shows the binodal curves obtained from Bleasdales equation. The effect of pH is clear: very small on the size of the heterogeneous region. This trend is in agreement with the experimental results of de Oliveira [12] and Martins [15]. Figure 1. Phase diagram of the PEG (1500) + di-potassium tartrate + water (3) two-phase system at T = 298.15 K and various pH (6.41, 7.74 and 9.05): (à ¢-  ) experimental binodal (6.41(pink), 7.74(blue) and 9.05 (green); (à ¢- ²) calculated binodal using Bleasdales equation (3). TLL and STL Tie line compositions are given in Table 4. Figure 2 presents the tie lines and the binodal curve together for the PEG + di-potassium tartrate + water system at 298.15 K. Figure2. Phase diagram of the PEG + di-potassim tartrate + water two-phase system at T = 298.15 K and pH 6.41 (a), 7.74 (b) and 9.05 (c) : (-à ¢- ²-) experimental binodal; ; (- -à ¢- - -) calculated by using eq. 4. Table 4. Phase composition, tie-line data and physical properties of PEG 1500 + di-potassium tartrate+ water aqueous two-phase system at 298.15 K and 0.1 MPa Total System (%mass) Top phase Bottom phase 6.41 17 20 10.55 33.70 1.1217 1.3922 23.50 4.65 1.1620 1.3703 31.80 2.24 17 21 10.21 35.50 1.1226 1.3936 24.01 4.31 1.1652 1.3705 34.10 2.26 18 20 9.50 39.57 1.1252 1.3965 25.01 3.70 1.1716 1.3710 39.07 2.31 18 21 9.01 42.65 1.1275 1.3985 26.01 3.17 1.1782 1.3717 42.97 2.32 7.74 17 20 9.01 35.77 1.1142 1.3919 23.15 5.29 1.1606 1.3710 33.62 2.14 17 21 8.30 39.31 1.1157 1.3932 24.01 4.74 1.1660 1.3714 37.96 2.20 18 20 7.80 42.04 1.1172 1.3947 24.70 4.32 1.1705 1.3718 41.33 2.23 18 21 7.20 45.58 1.1195 1.3966 26.01 3.62 1.1791 1.3727 45.97 2.23 9.05 17 20 4.01 39.51 1.0844 1.3831 25.18 6.76 1.1791 1.3781 39.00 1.54 17 21 3.30 41.88 1.0836 1.3835 27.01 5.80 1.1912 1.3796 43.17 1.52 18 20 3.08 42.66 1.0835 1.3836 28.01 5.34 1.1980 1.3806 44.87 1.49 18 21 2.80 43.67 1.0833 1.3838 28.80 4.99 1.2034 1.3814 46.59 1.48 The tie lines are determined by connecting each corresponding set of total, top, and bottom phase compositions. The coexisting phases are close in composition. A mass balance check was made between the initial mass of each component and the amounts in the bottom and top phases on the basis of equilibrium compositions. The mass of each phase was calculated from volume and density measurements. The relative error in the mass balance was less than 3 while those of the top phases ranged from 1.08 to 1.12 g/cm3. The density difference between the phases (ΆÃƒ Ã‚ ), increase with an increase in the TLL and slightly decrease with an increase in pH. From Figures 7, it is observed that the density differences between the phases show linear relationship with TLL. A comparable conduct was likewise depicted 31, 34, 35. Figure *. Relationship between density difference (ΆÃƒ Ã‚ ) and tie line length (TLL) for the PEG 1500 + di-potassium tartrate + water at different pH values. 3.4. Binodal curve and tie-line data correlation The coefficients of equation 4, along with the correspond